Not medical advice. Talk to your provider before using any peptide.
Full disclaimerPeptide Protocols
Every protocol is sourced from a specific person, paper, or label. Filter by goal, experience level, or confidence tier to find protocols that match where you are. Pick one, set a start date, and the schedule runs on your calendar.
14 researchers, clinicians, and public figures
Wolverine, GLOW, Never Get Sick, and more
All Protocols
Cheat Sheet Personal Stack
Ipamorelin + Tesamorelin + BPC-157 +2
Five peptides from the co-author of Jay Campbell's Peptide Cheat Sheet. Hunter Williams disclosed his personal protocol: Ipamorelin 300mcg, Tesamorelin 1mg, BPC-157 500mcg, TB-500 500mcg, and TA-1, all cycled 8 weeks on and 8 weeks off. The dosing lines up with the cheat sheet he co-wrote, which adds credibility. He also runs Tirzepatide 0.5mg three times per week but that sits outside the core stack. Budget $300-600/month.
Wolverine Stack
BPC-157 + TB-500 + Ipamorelin +2
Five peptides, three sub-stacks, and a torn hamstring that started it all. Ben Greenfield published full dosing for BPC-157, TB-500, ipamorelin, tesamorelin, and GHK-Cu after using the combo to recover from a persistent upper leg injury. The healing duo (BPC-157 + TB-500) can be run alone; the GH pair (ipamorelin + tesamorelin) adds recovery optimization at bedtime. Budget $400-800/month and plan for 4-12 weeks on, equal time off.
GLOW Protocol
GHK-Cu + BPC-157 + TB-500
Three peptides blended in a 5:1:1 ratio: GHK-Cu (50mg), BPC-157 (10mg), and TB-500 (10mg) in a single 70mg vial. Jay Campbell designed the GLOW protocol for tissue repair, gut healing, and skin rejuvenation. Available as a pre-blended vial or mixed individually. Daily injection, with at least 15 days off between cycles to prevent receptor desensitization. Beginner-friendly entry point.
Dr. Holtorf's Immune Modulation Protocol
Thymosin Alpha 1 + BPC-157 + Epithalon
Three peptides for immune modulation, gut healing, and telomere maintenance. TA-1 at clinical dose (1.6mg 2x/week), oral BPC-157 for gut health, and Epithalon in 10-day pulses. Note: Holtorf co-founded Integrative Peptides which sells oral BPC-157.
Healing Stack: BPC-157 + TB-500
BPC-157 + TB-500
Gold-standard healing combination. BPC-157 for local tissue repair + TB-500 for systemic healing. 6-week protocol with TB-500 loading then maintenance.
Dr. Koniver's GH Secretagogue Bedtime Stack
BPC-157 + Ipamorelin + Tesamorelin
One syringe, three peptides, taken at bedtime. Dr. Koniver confirmed on Huberman Lab Ep 197 that he combines BPC-157 (500mcg), ipamorelin (100mcg), and tesamorelin (2mg) in a single subcutaneous injection. Schedule is 5 days on, 2 off. No food for 45 minutes before. The single-syringe detail settled a long-running debate about whether these peptides could be mixed safely.
JF Tremblay's Anti-Aging Stack
Epithalon + BPC-157
A biochemist's anti-aging approach: Epithalon at ~3.3-5mg/day for 20-30 days (100mg total per cycle, 2-3x/year) plus BPC-157 in two modes: 10mg monthly high-dose pulse or 250mcg daily for 2 weeks on / 6 weeks off. Tremblay owns CanLab and has 30+ years in peptide manufacturing.
Dr. Edwin Lee's BPC-157 Knee Protocol (Clinical Trial)
BPC-157
The only peer-reviewed human BPC-157 clinical trial, published in Alternative Therapies (2021). Dr. Edwin Lee injected 4mg BPC-157 directly into knee joints. Seven out of 12 patients reported pain relief lasting 6+ months from a single injection. The route is intra-articular, not subcutaneous. This is a physician-administered procedure, not a self-injection protocol.
Huberman Recovery Protocol
BPC-157
Huberman used BPC-157 for an L5 disc compression from deadlifting and reported resolution in "approximately 2 injections" after massage, heat, and electrical stimulation had all failed. Dose is a discussed range (300-500mcg, 2-3 times per week), not his exact personal dose. Sourced from Huberman Lab Ep 170. Cycle 8 weeks on, 8-10 weeks off.
BPC-157 Conservative Healing Protocol
BPC-157
Conservative 6-week healing protocol using beginner-tier dosing. Inject near injury site for local repair or abdomen for systemic/gut effects.
12-Week Transformation
GHRP-6 + CJC-1295 (No DAC) + Ipamorelin +3
Six peptides across 5 timed injections per day for 12 weeks. Three GH secretagogues (GHRP-6 morning, ipamorelin pre-workout, GHRP-2 evening) plus CJC-1295 twice daily, AOD-9604 fasted, and TB-500 twice weekly. Aggressive stacking from Enhanced Athlete's founder.
30-Day Longevity Stack
SS-31 (Elamipretide) + Epithalon + Thymosin Alpha 1 +1
Four peptides, four aging mechanisms, 30 days. Ben Pakulski targets mitochondria with SS-31 (10mg daily), telomeres with Epithalon (5mg, 5x/week), immune function with TA-1 (1.5mg daily), and senescent cells with FOXO4-DRI (10mg EOD for 2 weeks). The FOXO4-DRI dose is 3.3x the standard aggressive tier. Budget $500-1,200 and consider starting with 1-2 peptides rather than all four simultaneously.
Never Get Sick Stack
TB-500 + Thymalin + LL-37
Three peptides, three different cycle lengths, one goal: stop getting sick. Greenfield pairs TB-500 (3 months on, 3 off) with Thymalin (3-10 day bursts every 6-12 months) and LL-37 (4-6 week antimicrobial cycles). Each targets a different layer of immune defense. Warning on LL-37: Herxheimer reactions are common in the first two weeks if you have SIBO or gut dysbiosis.
Dr. Edwin Lee's Longevity Protocol
Epithalon + Thymosin Alpha 1
Epithalon at 5mg daily for 10 days (2x/year) as his "#1 longevity peptide," plus TA-1 at a lower-than-standard 250mcg maintenance dose (1-2x/week, daily when sick). Discussed on the Dr. Hyman Show podcast.
Bryan Johnson's Thymus Rejuvenation Protocol
Epithalon + Thymosin Alpha 1
Bryan Johnson's thymus rejuvenation protocol from Project Blueprint. Epithalon (10mg/day) and Thymosin Alpha-1 (1.5mg/day) injected IM for 5 consecutive days, then nothing for 6 months. Follows the Khavinson protocol. Dosing sourced from tweets; the original post had ambiguous wording that the community later resolved. Budget $100-250 per pulse cycle.
TB-500 Loading + Maintenance Protocol
TB-500
Standard loading + maintenance cycle: 4 weeks at full dose (2x/week), then 4 weeks maintenance at half dose (1x/week). Systemic — injection site does not matter.
Jay Campbell's Beginner GH Stack
CJC-1295 (No DAC) + Ipamorelin
Campbell's recommended starting point for newcomers. CJC-1295 (no DAC) plus ipamorelin at bedtime, 5 on / 2 off, 8 weeks on and 8 off. He calls it "easy to purchase from a reliable supplier at a reasonable cost and extensively studied." The same combo shows up in both his muscle growth and fat loss stack lists, which tells you something about its versatility.
Peter Attia's GLP-1 Clinical Perspective
Semaglutide + Tirzepatide
Peter Attia prescribes GLP-1 agonists clinically and flagged the lean mass loss problem early: 39% of weight lost in the STEP 1 trial was lean tissue. His protocol follows standard FDA titration (0.25mg to 1mg+ weekly) but pairs it with resistance training and 1g protein per pound of bodyweight. This is a prescriber's perspective, not personal biohacking. DEXA scans and metabolic panels required.
GH Replication Stack
CJC-1295 (DAC) + MK-677 (Ibutamoren)
Derek's approach to replicating pharma-grade GH IGF-1 levels without actual GH. CJC-1295 DAC at 2.5mg twice weekly (Monday and Thursday) plus MK-677 at 25mg daily oral, both at bedtime. Target: IGF-1 around 500 ng/mL. The CJC-1295 DAC dose exceeds standard ranges. Regular IGF-1 bloodwork and fasting glucose monitoring required. Advanced users only.
Semaglutide Microdose
Semaglutide
Semaglutide at 0.025-0.05mg weekly, roughly 1/10th the standard starting dose. Dr. Tyna Moore's philosophy: "Dose up to appetite control, not suppression." Exact microgram dose is not publicly verified; the approach and philosophy come from multiple interviews including Ben Greenfield Life. She treats GLP-1 as scaffolding for building metabolic habits, not a permanent prescription.
Semaglutide Titration Protocol (Ozempic/Wegovy)
Semaglutide
FDA-standard weekly titration from 0.25mg to 2.4mg over 16+ weeks. Hold current dose for an extra 4 weeks if GI side effects are severe.
Bryan Johnson's Tirzepatide Microdose (DISCONTINUED)
Tirzepatide
Three weeks, then done. Johnson tried tirzepatide at 0.5mg weekly (1/5th the standard starting dose) and halted when his data turned negative: resting heart rate up 3bpm, HRV down 7 points, sleep quality down 10%. He already had optimal metabolic markers, so the risk outweighed any benefit. This is some of the best-documented peptide discontinuation data available, with exact biomarker numbers attached.
Tirzepatide Titration Protocol (Mounjaro/Zepbound)
Tirzepatide
FDA-standard weekly titration from 2.5mg to 15mg. Many maintain at 7.5-10mg without reaching max dose.
Huberman Sermorelin Protocol (DISCONTINUED)
Sermorelin
Two years on sermorelin for deep sleep, then he stopped. Huberman tracked everything with Eight Sleep and WHOOP; the data showed REM sleep depletion and elevated PSA (prostate-specific antigen). He disclosed this on Ep 170 as cautionary data. Dose is a discussed range, not his exact personal dose. This protocol is discontinued, but the discontinuation data is what makes it worth studying.
Bryan Johnson's Cerebrolysin Cognitive Protocol
Cerebrolysin
Bryan Johnson called cerebrolysin "the best subjective neuro-enhancer" he's tried, then added that it produced no measurable biomarker improvement. Phase 1: 5mL IM every other day (10 injections over 20 days). Phase 2: 5mL IM daily for 3 months. Porcine-derived, so not suitable for pork allergies. The gap between subjective experience and objective data is the interesting part here.
Retatrutide Titration Protocol (Triple G)
Retatrutide
Phase 3 trial protocol: weekly titration from 1mg to 12mg. Triple GLP-1/GIP/Glucagon agonist with up to 24% weight loss in clinical trials.
Test protocol
Survodutide + Survodutide
This is a description of the protocol
How Protocols Work
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