Peptide Schedule

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CommunityAnti-AgingVerified Dosing

Ben Pakulski's 30-Day Longevity Stack

Four peptides, four aging mechanisms, 30 days. Ben Pakulski targets mitochondria with SS-31 (10mg daily), telomeres with Epithalon (5mg, 5x/week), immune function with TA-1 (1.5mg daily), and senescent cells with FOXO4-DRI (10mg EOD for 2 weeks). The FOXO4-DRI dose is 3.3x the standard aggressive tier. Budget $500-1,200 and consider starting with 1-2 peptides rather than all four simultaneously.

4 phases·4 weeks·$500-$1200/mo·advanced
Anti-Aging

For targeting four aging mechanisms simultaneously over 30 days: mitochondria, telomeres, immune function, and senescent cell clearance.

What This Protocol Does

SS-31 stabilizes mitochondrial cardiolipin to improve ATP production (10mg daily). Epithalon activates telomerase for telomere maintenance (5mg, 5 days per week). TA-1 supports T-cell production and immune function (1.5mg daily). FOXO4-DRI triggers apoptosis of senescent cells (10mg every other day for 2 weeks, then stops). Each peptide targets a different aging pathway.

Who Should Use This

Advanced users with prior peptide experience and a budget of $500-1,200 for the 30-day protocol. Not a beginner stack. Consider starting with 1-2 peptides rather than all four at once. The FOXO4-DRI dose (10mg EOD) is 3.3x the standard aggressive tier, so prior senolytic experience is recommended. Consult a physician, especially if you have cancer history or immune disorders.

What the Research Says

SS-31 (elamipretide) has Phase 2/3 clinical trial data for mitochondrial myopathy and Barth syndrome. It stabilizes cardiolipin in the inner mitochondrial membrane (Szeto, PMID 24606927). Epithalon follows the Khavinson protocol, with published data on telomerase activation in human cell cultures and improved survival in elderly subjects (Khavinson, PMID 14523363). Thymosin alpha-1 is approved in 30+ countries for hepatitis B/C and has clinical data showing T-cell and NK cell activation (Tuthill et al., PMID 10813511). FOXO4-DRI is a senolytic peptide tested in aged mice, where it cleared senescent cells and restored fitness markers (Baar et al., PMID 28340339). No human trials exist for FOXO4-DRI.

Important Notices

  • FOXO4-DRI at 10mg EOD is above standard longevity practitioner dosing
  • SS-31 dose is lower than our DB tiers (10mg vs our 20mg beginner)
  • 4 peptides simultaneously is advanced — consider starting with 1-2
Dosing Confidence
Verified Dosing
Experience Level
advanced
Est. Monthly Cost
$500-$1200/mo
Cycling
30-day protocol. FOXO4-DRI runs 2 weeks then stops. Others run full 30 days.

Protocol Overview

4 weeks total
SS-31 (Elamipretide)
10mg
5w
Epithalon
5mg
5w
Thymosin Alpha 1
1.5mg
5w
FOXO4-DRI
10mg
5w
4 Sub-Stacks — each can be started independently

Mitochondrial Support

Standalone

SS-31 (Elamipretide)

Daily

Telomere / Sleep

Standalone

Epithalon

5x/week

Immune Modulation

Standalone

Thymosin Alpha 1

Daily

Senolytic

Standalone

FOXO4-DRI

EOD for 2 weeks, then off

Dosing Details

PeptideDoseFrequencyRouteWeeks
10mgDailysubcutaneousWeeks 1-5
5mgCustom SchedulesubcutaneousWeeks 1-5
1,500mcgDailysubcutaneousWeeks 1-5
10mgEvery Other DaysubcutaneousWeeks 1-2

SS-31 (Elamipretide): 10mg daily SC for 30 days. Stabilizes mitochondrial cardiolipin.

Epithalon: 5mg, 5x/week for 30 days. Telomerase activator.

Thymosin Alpha 1: 1.5mg daily for 30 days. Immune modulation.

FOXO4-DRI: 10mg EOD for 2 weeks then stop. Targets senescent cells.

Metrics Tracked

Skin Quality
Scale 0-10 · weekly
Energy Level
Scale 0-10 · daily
Recovery
Scale 0-10 · weekly

Schedule This Protocol

Set your start date and track every dose in your calendar.

Use This Protocol

Peptide Profiles & Calculators

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