Ben Pakulski's 30-Day Longevity Stack
Peptide Schedule Research TeamReviewed Apr 2026
Four peptides, four aging mechanisms, 30 days. Ben Pakulski targets mitochondria with SS-31 (10mg daily), telomeres with Epithalon (5mg, 5x/week), immune function with TA-1 (1.5mg daily), and senescent cells with FOXO4-DRI (10mg EOD for 2 weeks). The FOXO4-DRI dose is 3.3x the standard aggressive tier. Budget $500-1,200 and consider starting with 1-2 peptides rather than all four simultaneously.
Protocol Source
Important Notices
- ⚠FOXO4-DRI at 10mg EOD is above standard longevity practitioner dosing
- ⚠SS-31 dose is lower than our DB tiers (10mg vs our 20mg beginner)
- ⚠4 peptides simultaneously is advanced — consider starting with 1-2
Sub-Stacks
This protocol contains 4 groups. Each can be started independently unless noted.
Mitochondrial Support
ATP production via cardiolipin stabilization
Timing: Daily
Telomere / Sleep
Telomerase activation
Timing: 5x/week
Immune Modulation
Immune system support
Timing: Daily
Senolytic
Clear senescent cells
Timing: EOD for 2 weeks, then off
Protocol Phases
10mg daily SC for 30 days. Stabilizes mitochondrial cardiolipin.
5mg, 5x/week for 30 days. Telomerase activator.
1.5mg daily for 30 days. Immune modulation.
10mg EOD for 2 weeks then stop. Targets senescent cells.
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Use This ProtocolRelated Protocols
Bryan Johnson's Thymus Rejuvenation Protocol
Bryan Johnson's thymus rejuvenation protocol from Project Blueprint. Epithalon (10mg/day) and Thymosin Alpha-1 (1.5mg/day) injected IM for 5 consecutive days, then nothing for 6 months. Follows the Khavinson protocol. Dosing sourced from tweets; the original post had ambiguous wording that the community later resolved. Budget $100-250 per pulse cycle.
via Bryan JohnsonDr. Edwin Lee's Longevity Protocol
Epithalon at 5mg daily for 10 days (2x/year) as his "#1 longevity peptide," plus TA-1 at a lower-than-standard 250mcg maintenance dose (1-2x/week, daily when sick). Discussed on the Dr. Hyman Show podcast.
via Dr. Edwin LeeJF Tremblay's Anti-Aging Stack
A biochemist's anti-aging approach: Epithalon at ~3.3-5mg/day for 20-30 days (100mg total per cycle, 2-3x/year) plus BPC-157 in two modes: 10mg monthly high-dose pulse or 250mcg daily for 2 weeks on / 6 weeks off. Tremblay owns CanLab and has 30+ years in peptide manufacturing.
Not medical advice. Talk to your provider before using any peptide.
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