Dr. Holtorf's Immune Modulation Protocol

Conservative 6-week healing protocol using beginner-tier dosing. Inject near injury site for local repair or abdomen for systemic/gut effects.

Standard loading + maintenance cycle: 4 weeks at full dose (2x/week), then 4 weeks maintenance at half dose (1x/week). Systemic — injection site does not matter.

Gold-standard healing combination. BPC-157 for local tissue repair + TB-500 for systemic healing. 6-week protocol with TB-500 loading then maintenance.

Five peptides, three sub-stacks, and a torn hamstring that started it all. Ben Greenfield published full dosing for BPC-157, TB-500, ipamorelin, tesamorelin, and GHK-Cu after using the combo to recover from a persistent upper leg injury. The healing duo (BPC-157 + TB-500) can be run alone; the GH pair (ipamorelin + tesamorelin) adds recovery optimization at bedtime. Budget $400-800/month and plan for 4-12 weeks on, equal time off.

via Ben Greenfield

Three peptides, three different cycle lengths, one goal: stop getting sick. Greenfield pairs TB-500 (3 months on, 3 off) with Thymalin (3-10 day bursts every 6-12 months) and LL-37 (4-6 week antimicrobial cycles). Each targets a different layer of immune defense. Warning on LL-37: Herxheimer reactions are common in the first two weeks if you have SIBO or gut dysbiosis.

via Ben Greenfield

One syringe, three peptides, taken at bedtime. Dr. Koniver confirmed on Huberman Lab Ep 197 that he combines BPC-157 (500mcg), ipamorelin (100mcg), and tesamorelin (2mg) in a single subcutaneous injection. Schedule is 5 days on, 2 off. No food for 45 minutes before. The single-syringe detail settled a long-running debate about whether these peptides could be mixed safely.

via Dr. Craig Koniver

The only peer-reviewed human BPC-157 clinical trial, published in Alternative Therapies (2021). Dr. Edwin Lee injected 4mg BPC-157 directly into knee joints. Seven out of 12 patients reported pain relief lasting 6+ months from a single injection. The route is intra-articular, not subcutaneous. This is a physician-administered procedure, not a self-injection protocol.

via Dr. Edwin Lee

Huberman used BPC-157 for an L5 disc compression from deadlifting and reported resolution in "approximately 2 injections" after massage, heat, and electrical stimulation had all failed. Dose is a discussed range (300-500mcg, 2-3 times per week), not his exact personal dose. Sourced from Huberman Lab Ep 170. Cycle 8 weeks on, 8-10 weeks off.

via Andrew Huberman

Three peptides blended in a 5:1:1 ratio: GHK-Cu (50mg), BPC-157 (10mg), and TB-500 (10mg) in a single 70mg vial. Jay Campbell designed the GLOW protocol for tissue repair, gut healing, and skin rejuvenation. Available as a pre-blended vial or mixed individually. Daily injection, with at least 15 days off between cycles to prevent receptor desensitization. Beginner-friendly entry point.

via Jay Campbell

Two years on sermorelin for deep sleep, then he stopped. Huberman tracked everything with Eight Sleep and WHOOP; the data showed REM sleep depletion and elevated PSA (prostate-specific antigen). He disclosed this on Ep 170 as cautionary data. Dose is a discussed range, not his exact personal dose. This protocol is discontinued, but the discontinuation data is what makes it worth studying.

via Andrew Huberman

Bryan Johnson called cerebrolysin "the best subjective neuro-enhancer" he's tried, then added that it produced no measurable biomarker improvement. Phase 1: 5mL IM every other day (10 injections over 20 days). Phase 2: 5mL IM daily for 3 months. Porcine-derived, so not suitable for pork allergies. The gap between subjective experience and objective data is the interesting part here.

via Bryan Johnson

Five peptides from the co-author of Jay Campbell's Peptide Cheat Sheet. Hunter Williams disclosed his personal protocol: Ipamorelin 300mcg, Tesamorelin 1mg, BPC-157 500mcg, TB-500 500mcg, and TA-1, all cycled 8 weeks on and 8 weeks off. The dosing lines up with the cheat sheet he co-wrote, which adds credibility. He also runs Tirzepatide 0.5mg three times per week but that sits outside the core stack. Budget $300-600/month.

Six peptides across 5 timed injections per day for 12 weeks. Three GH secretagogues (GHRP-6 morning, ipamorelin pre-workout, GHRP-2 evening) plus CJC-1295 twice daily, AOD-9604 fasted, and TB-500 twice weekly. Aggressive stacking from Enhanced Athlete's founder.

via Tony Huge