Not medical advice. Talk to your provider before using any peptide.
Full disclaimerGLOW Peptide Protocol — GHK-Cu, BPC-157 & TB-500 Blend by Jay Campbell
Three peptides blended in a 5:1:1 ratio: GHK-Cu (50mg), BPC-157 (10mg), and TB-500 (10mg) in a single 70mg vial. Jay Campbell designed the GLOW protocol for tissue repair, gut healing, and skin rejuvenation. Available as a pre-blended vial or mixed individually. Daily injection, with at least 15 days off between cycles to prevent receptor desensitization. Beginner-friendly entry point.
Peptide Schedule Research TeamReviewed Apr 2026
For tissue repair, gut healing, and skin rejuvenation using a pre-blended 5:1:1 ratio of GHK-Cu, BPC-157, and TB-500.
What This Protocol Does
Three peptides blended in a single 70mg vial at a 5:1:1 ratio: GHK-Cu (50mg), BPC-157 (10mg), and TB-500 (10mg). One daily injection delivers all three. GHK-Cu drives collagen remodeling and skin repair. BPC-157 handles tissue repair and gut healing. TB-500 provides systemic anti-inflammatory effects. The pre-blended format eliminates the need to reconstitute and draw from three separate vials.
Who Should Use This
Beginners looking for a simple daily injection protocol with multiple benefits. The pre-blended vial makes this the easiest multi-peptide protocol to run. Avoid with active cancer (all three promote angiogenesis). Not for competitive athletes (WADA prohibited). Take at least 15 days off between cycles to prevent receptor desensitization. Budget varies by supplier since it's sold as a pre-made blend.
What the Research Says
GHK-Cu has 40+ published studies on wound healing, collagen synthesis, and anti-inflammatory effects (Pickart et al., PMID 22585065). BPC-157 has 100+ preclinical studies on tissue repair and gut protection (Sikiric et al., PMID 9073154). TB-500 (thymosin beta-4) has Phase 2 data for cardiac repair and wound healing (Crockford, PMID 17303110). No published studies test the specific 5:1:1 combination. Jay Campbell designed the ratio based on clinical experience and community feedback.
Important Notices
- Promotes angiogenesis — avoid with active cancer
- Competitive athletes: WADA prohibited
- Take at least 15 days off between cycles to prevent receptor desensitization
Protocol Overview
GLOW Blend (5:1:1)
GHK-Cu + BPC-157 + TB-500
Daily injection
Contraindications
- Active cancer
- Pregnancy or nursing
- Blood thinners without supervision
- Competitive athletes (WADA)
Dosing Details
| Peptide | Dose | Frequency | Route | Weeks |
|---|---|---|---|---|
| 1,670mcg | Daily | subcutaneous | Week 1+ | |
| 330mcg | Daily | subcutaneous | Week 1+ | |
| 330mcg | Daily | subcutaneous | Week 1+ |
GHK-Cu: ~1.67mg per injection from 70mg pre-blended vial (50:10:10 ratio). 5:1:1 GHK-Cu:TB-500:BPC-157.
BPC-157: ~330mcg per injection from pre-blended vial.
TB-500: ~330mcg per injection from pre-blended vial.
Metrics Tracked
Schedule This Protocol
Set your start date and track every dose in your calendar.
Peptide Profiles & Calculators
Related Healing & Recovery Protocols
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Conservative 6-week healing protocol using beginner-tier dosing. Inject near injury site for local repair or abdomen for systemic/gut effects.
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Standard loading + maintenance cycle: 4 weeks at full dose (2x/week), then 4 weeks maintenance at half dose (1x/week). Systemic — injection site does not matter.
Healing Stack: BPC-157 + TB-500
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Gold-standard healing combination. BPC-157 for local tissue repair + TB-500 for systemic healing. 6-week protocol with TB-500 loading then maintenance.
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Five peptides, three sub-stacks, and a torn hamstring that started it all. Ben Greenfield published full dosing for BPC-157, TB-500, ipamorelin, tesamorelin, and GHK-Cu after using the combo to recover from a persistent upper leg injury. The healing duo (BPC-157 + TB-500) can be run alone; the GH pair (ipamorelin + tesamorelin) adds recovery optimization at bedtime. Budget $400-800/month and plan for 4-12 weeks on, equal time off.
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Dr. Koniver's GH Secretagogue Bedtime Stack
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One syringe, three peptides, taken at bedtime. Dr. Koniver confirmed on Huberman Lab Ep 197 that he combines BPC-157 (500mcg), ipamorelin (100mcg), and tesamorelin (2mg) in a single subcutaneous injection. Schedule is 5 days on, 2 off. No food for 45 minutes before. The single-syringe detail settled a long-running debate about whether these peptides could be mixed safely.
