How many units of TB-500 should I draw?

It depends on your vial size, BAC water, and dose. For a 2mg vial with 1mL BAC water, a beginner dose of 2000mcg is typically 100 units on a U-100 syringe. Use the calculator above for your exact numbers.

How much BAC water should I add to TB-500?

The standard amount is 1mL of bacteriostatic water for a 2mg vial. More water gives a more dilute solution — easier to measure small doses. Less water means fewer injections per vial. Adjust the BAC water slider in the calculator to see how it changes your syringe units.

How long does a TB-500 vial last?

At a beginner dose of 2000mcg 2x/week, a 2mg vial lasts about 1 doses. The calculator shows exact doses per vial for your selected tier.

What syringe should I use for TB-500?

Most people use a U-100 insulin syringe (1mL / 100 units). For very small doses, a U-50 or U-30 syringe gives better precision. The calculator adjusts units automatically for whichever syringe you pick.

How should I store TB-500 after reconstitution?

Store reconstituted TB-500 at 2-8°C (36-46°F). Refrigerate, use within 3-4 weeks. Before reconstitution: refrigerate at 2-8°c, stable up to 24 months.

How does TB-500 compare to similar peptides?

TB-500 has 1 head-to-head comparison available: BPC-157. See the full comparison pages for dosing differences, mechanisms, and which one fits your goals.

What is the recommended TB-500 dosage?

Loading phase: 2,000-2,500 mcg subcutaneous, twice per week for 4-6 weeks. Maintenance: 2,000-2,500 mcg once per week or every two weeks. The loading phase front-loads systemic thymosin beta-4 levels. Some community protocols run 5,000 mcg twice weekly for acute injuries.

Not medical advice. Talk to your provider before using any peptide.

Full disclaimer

Peptide Schedule Research TeamReviewed Apr 202610 Citations

Healing & RecoveryPreclinicalGrade C

Accelerated HealingAnti-InflammatoryCellular Regeneration

TB-500 Dosage Calculator

5 h half-life·Injection·6w on / 2w off

TB-500 is the most popular systemic healing peptide in the research community, with 500+ forum threads and a 4.1/5 sentiment score. Backed by strong preclinical wound-healing data but zero completed human trials for the fragment itself. Inject anywhere; it reaches the injury regardless of site.

Used by Greenfield, Campbell + 7 more

Hot500+172High

13 referencesVerified 2026-04-09

Vial Size

BAC Water (mL)

Dosing Level

2mg · 2x/week

Custom Dose (mcg) — optional

Dose by Body Weight

Syringe Type

100.0 units

100 units (1mL)

Concentration

2,000

mcg/mL

Draw Volume

1.000

Syringe Units

100.0

units

Doses / Vial

doses

Summary: Add 1mL BAC water to your 2mg vial. Draw to 100.0 units on a U-100 syringe for a 2mg dose. This vial will last 1 doses.

Schedule This Protocol

2mg 2x/week — draw 100.0 units on U-100 syringe

Subcutaneous or Intramuscular. Typical beginner frequency: 2x/week.At beginner dose (2000mcg) with a 2mg/1mL vial: draw to the 100-unit mark on a U-100 syringe.

After reconstituting, store at 2-8°C (36-46°F) (refrigerate, use within 3-4 weeks).

View side effects and safety warnings →

Quick-Start: Reconstitution

1Reconstitute: add 1 mL bacteriostatic water to a 5 mg vial. Aim the stream against the glass wall, not directly onto the powder. Swirl gently until dissolved. Concentration: 5000 mcg per mL.
2Draw your dose using a 100-unit insulin syringe. Beginner (2000 mcg): draw to the 40-unit mark. Moderate (5000 mcg): draw to the 100-unit mark (full syringe). For a 10 mg vial reconstituted with 1 mL BAC water (10,000 mcg/mL), beginner dose is 20 units.
3Inject subcutaneously. Pinch a fold of skin on the abdomen or thigh. Insert a 27 to 29 gauge, 0.5 inch needle at a 45-degree angle. Push the plunger slowly.

Full injection guide →

TB-500 Dosing Protocol

CommunityCommunity dosing consensus from peptide research communities

Level	Dose / Injection	Frequency
Beginner	2mg	2x/week
Moderate	2,500mcg	2x/week
Aggressive	5mg	2x/week

Note:

All TB-500 dosing is community-driven. No human dose-finding study exists for the fragment. Beginner: 2000 mcg (2 mg) twice weekly. Moderate: 5000 mcg (5 mg) twice weekly. Aggressive: 7500 mcg (7.5 mg) twice weekly. Reconstitution math for a 5 mg vial with 1 mL bacteriostatic water: concentration is 5000 mcg per mL. Each 10 units on a 100-unit insulin syringe equals 500 mcg. So 2000 mcg (beginner dose) is 40 units. For a 10 mg vial with 1 mL BAC water: 10,000 mcg per mL, meaning 20 units for 2000 mcg. The loading phase matters more than anything else. Skipping it is the single most common reason for disappointing results. You need 2 injections per week for at least 4 to 6 weeks to build adequate tissue-level concentrations. Going straight to once-weekly maintenance is an underdose. Don't train through the injury during a TB-500 protocol. The community figured this out quickly: TB-500 accelerates repair, but it doesn't protect against re-injury from continued loading of the damaged tissue. Relative rest of the injured structure speeds outcomes. Injection site does not need to be near the injury. Abdomen or thigh, alternating sides.

Dosing based on Community dosing consensus from peptide research communities — 13 published references.View all sources →

Verify your calculation

Cross-check your TB-500 reconstitution math with AI

TB-500 Cost

Compounded

Removed from FDA Category 2 on April 15, 2026. PCAC review begins July 2026. Compounding pharmacy access projected late 2026 to mid-2027. Not yet available from US 503A/503B compounding pharmacies.

Generic / Research

2 mg research vial: ~$23–$35 (e.g., Sigma Labs ~$23.75) | 5 mg vial: ~$50–$78 (e.g., Core Peptides $78, Ignite Peptides lower end) | 10 mg vial: ~$69–$140 (e.g., US Chem Labs ~$70, Core Peptides $140, SwissChems in range): wide price spread reflects real quality variation | BPC-157/TB-500 blend 10 mg vials: ~$60–$100 from multiple vendors | Monthly protocol cost at conservative loading (2 mg 2×/week, ~16 mg/month): approximately $115–$190 using 5 mg vials

Pricing updated 2026-04-09

Prices are estimates and vary by source, location, and prescription status.Full pricing breakdown →

TB-500 Pharmacokinetics

Pharmacokinetics — Active Dose Over Time

t½ = ~5 hours plasma (functional tissue activity persists 7-10 days)

After 1 half-life (9d): 50% remainsAfter 2 half-lives (17d): 25% remainsAfter 3 half-lives (26d): 12.5% remains

At a 2.5mg dose: 50% = 1.3mg remaining after 9d. Recommended frequency: 2x/week.

Disclaimer: This curve is a simplified first-order exponential decay model. Actual pharmacokinetics vary based on injection site, individual metabolism, body composition, and other factors. Half-life values are approximate and based on available preclinical and clinical literature. Many research peptides lack formal human pharmacokinetic studies. This is for educational purposes only — not medical advice.