Not medical advice. Talk to your provider before using any peptide.
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Bryan Johnson
Entrepreneur & Longevity Pioneer
Sold Braintree/Venmo for $800M, then turned himself into a longevity experiment. Bryan Johnson spends $2M+ per year on anti-aging protocols tracked by a team of 30+ doctors through Project Blueprint. Every peptide trial gets biomarker data before, during, and after. He halted tirzepatide after 3 weeks when his resting heart rate climbed 3bpm and HRV dropped 7 points. That kind of discontinuation data, with exact numbers attached, is rare. Four protocols on Peptide Schedule, including two he stopped.
“Peptides are effective when used precisely. However, there is limited research for many peptides, so do your research, measure, and use a reputable supplier.”
— Bryan Johnson
Bryan Johnson treats his body like a dataset. After selling Braintree/Venmo for $800M, he launched Project Blueprint and now spends $2M+ per year on longevity protocols tracked by 30+ doctors. Every intervention gets biomarker data before, during, and after. His peptide approach follows the same logic: "Peptides are effective when used precisely. However, there is limited research for many peptides, so do your research, measure, and use a reputable supplier."
The discontinuation data is what stands out. Johnson halted tirzepatide after just 3 weeks when his resting heart rate climbed 3bpm, HRV dropped 7 points, and sleep quality fell 10%. He published those exact numbers. He also reported that cerebrolysin was his "best subjective neuro-enhancer" but produced no measurable biomarker improvement. That kind of honest negative reporting is rare.
Four protocols are tracked on this page (2 active, 2 discontinued). Dosing sourced from tweets with specific post IDs. Confidence tier is 2 for all; tweet-sourced data carries less weight than published articles or clinical studies.
Protocols by Bryan
3 disclosed protocols with verified dosing details.
Bryan Johnson's Thymus Rejuvenation Protocol
Epithalon + Thymosin Alpha 1
Bryan Johnson's thymus rejuvenation protocol from Project Blueprint. Epithalon (10mg/day) and Thymosin Alpha-1 (1.5mg/day) injected IM for 5 consecutive days, then nothing for 6 months. Follows the Khavinson protocol. Dosing sourced from tweets; the original post had ambiguous wording that the community later resolved. Budget $100-250 per pulse cycle.
Bryan Johnson's Cerebrolysin Cognitive Protocol
Cerebrolysin
Bryan Johnson called cerebrolysin "the best subjective neuro-enhancer" he's tried, then added that it produced no measurable biomarker improvement. Phase 1: 5mL IM every other day (10 injections over 20 days). Phase 2: 5mL IM daily for 3 months. Porcine-derived, so not suitable for pork allergies. The gap between subjective experience and objective data is the interesting part here.
Bryan Johnson's Tirzepatide Microdose (DISCONTINUED)
Tirzepatide
Three weeks, then done. Johnson tried tirzepatide at 0.5mg weekly (1/5th the standard starting dose) and halted when his data turned negative: resting heart rate up 3bpm, HRV down 7 points, sleep quality down 10%. He already had optimal metabolic markers, so the risk outweighed any benefit. This is some of the best-documented peptide discontinuation data available, with exact biomarker numbers attached.
Source Timeline
Verified sources where Bryan discussed peptide protocols.
Discontinued: raised RHR +3bpm, lowered HRV -7pts, sleep quality -10%
5mL IM daily — "best subjective neuro-enhancer" but no measurable biomarker effect
Exact dosing: 10mg/day IM x5 days every 6 months (Khavinson protocol)
Protocols shown here are based on publicly disclosed information from Bryan Johnson. They are reproduced for educational purposes only and are not endorsements. Always consult a healthcare provider before starting any peptide protocol.