How many units of Substance P should I draw?

It depends on your vial size, BAC water, and dose. For a 2mg vial with 2mL BAC water, a beginner dose of 50mcg is typically 5 units on a U-100 syringe. Use the calculator above for your exact numbers.

How much BAC water should I add to Substance P?

The standard amount is 2mL of bacteriostatic water for a 2mg vial. More water gives a more dilute solution, so the same dose fills more of the syringe and is easier to measure. Less water makes the draw smaller and harder to read. The number of doses per vial does not change either way, since that depends only on vial size and dose. Adjust the BAC water slider in the calculator to see how it changes your syringe units.

How long does a Substance P vial last?

At a beginner dose of 50mcg single dose (local injection near wound site), a 2mg vial lasts about 40 doses. The calculator shows exact doses per vial for your selected tier.

What syringe should I use for Substance P?

Most people use a 1mL U-100 insulin syringe (100 units). For very small doses, a 0.5mL or 0.3mL barrel is easier to read. All three are U-100 graduated, so the number of units to draw is the same on each. Veterinary U-40 syringes are a different scale and the calculator adjusts for that separately.

How should I store Substance P after reconstitution?

Store reconstituted Substance P at -20°C (long-term) or 2-8°C (short-term). Refrigerate at 2-8°C, use within 7 days. Aliquot to avoid freeze-thaw cycles.. Before reconstitution: store at -20°c for long-term stability, up to 2 years. stable at 4°c for several months..

If Substance P breaks down in 1-2 minutes, how can a daily SubQ injection do anything?

Great question, and honestly, it's the core pharmacological limitation. With degradation enzymes abundant in tissue fluid, a SubQ dose is mostly destroyed locally before reaching systemic circulation. Any effect from SubQ would be primarily local, near the injection site. Clinical research uses continuous IV infusion for systemic effects or topical application for corneal wound healing. Daily SubQ for systemic benefit has no published support.

Not medical advice. Talk to your provider before using any peptide.

Full disclaimer

Peptide Schedule Research TeamReviewed Apr 20269 Citations

Substance P Dosage Calculator

Turns your vial size, BAC water, and dose into exact syringe units. Free, no signup.

ImmuneResearchGrade C·~1-2 minutes half-life·Injection·4w on / 4w off

13 refsVerified 2026-04-07

Calculate your dose

Adjust vial, water, and dose — answer updates live

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Vial Size

BAC Water (mL)

Dose Tier

50mcg · Single dose (local injection near wound site)

Custom Dose (mcg) optional

Syringe

All three take the same units for the same dose. A smaller barrel just spreads it over more space.

Draw to 5.0 units= 0.050 mL

on a U-100 syringe for a 50mcg dose

Concentration

1,000

mcg/mL

Doses / vial

Cost / dose

Your mixing recipe

Add 2 mL BAC water to your 2 mg vial. Draw to 5.0 units on a U-100 syringe for a 50mcg dose. This vial will last 40 doses.

Double-check with AI Substance P profile

Copy link saves these numbers in the address. Anyone who opens it sees the same draw.

Scheduling does not apply to Substance P

There is no repeating self-administered schedule to plan for it, so the protocol builder leaves it out. The calculator above still works out the draw. Read the Substance P profile

Scheduling does not apply to Substance P

There is no repeating self-administered schedule to plan for it, so the protocol builder leaves it out. The calculator above still works out the draw. Read the Substance P profile

Cycle Length (weeks)

Price Per Vial ($) — optional

Open Substance P details

Side effects, protocols by goal, storage, and the sources behind these dosing numbers.

About Substance P

Substance P activates NK1R to mobilize bone marrow stem cells and stimulate wound repair in animal models (Kim et al., Nature Medicine, PMID 19270709). A tissue half-life of 1 to 2 minutes and zero human wound healing trials limit it to preclinical research settings.

NeuropeptidePain SignalingNeurogenic InflammationImmune ModulationWound HealingNK1 Receptor

NicheNear-zero self-admin20,000Moderate

Subcutaneous / Intravenous (research settings). Typical beginner frequency: single dose (local injection near wound site).At beginner dose (50mcg) with a 2mg/2mL vial: draw to the 5-unit mark on a U-100 syringe.

After reconstituting, store at -20°C (long-term) or 2-8°C (short-term) (refrigerate at 2-8°c, use within 7 days. aliquot to avoid freeze-thaw cycles.).

View side effects and safety warnings →

Quick-Start: Reconstitution

1Confirm your source. Verify the certificate of analysis shows at least 95% HPLC purity and correct molecular weight (1,347.63 Da). Research-grade only.
2Reconstitute. Add 2 mL bacteriostatic water to a 2 mg vial. Swirl gently. Do not shake or vortex. Final concentration: 1 mcg per uL.
3Draw your dose. For a 50 mcg beginner dose, draw to the 5-unit mark on a U-100 insulin syringe. For 100 mcg (moderate), draw to 10 units. For 200 mcg (aggressive), draw to 20 units.

Full injection guide→

Need a different vial size? Open the universal Substance P reconstitution calculator to enter any vial mg + BAC water mL.

Substance P Dosing Protocol

SpeculativeVery limited human dosing data: extrapolated from research studies

Level	Dose / Injection	Frequency
Beginner	50mcg	Single dose (local injection near wound site)
Moderate	100mcg	Single dose (local injection near wound site)
Aggressive	200mcg	Single dose (local injection near wound site)

Note:

Start with two practical realities. First: Substance P is not sold by consumer peptide vendors. You need a research-grade source (Sigma-Aldrich S6883 or Tocris #1156), and most require institutional purchasing credentials. Second: the 1 to 2 minute tissue half-life means distant subcutaneous injection (abdomen for a knee wound, for example) is pharmacologically pointless. Inject directly at or immediately adjacent to the target tissue. Reconstitution math for a 2 mg vial: add 2 mL bacteriostatic water. Concentration equals 1 mg/mL, or 1 mcg per uL. A 50 mcg dose equals 50 uL, which is 5 units on a U-100 insulin syringe. A 100 mcg dose equals 10 units. A 200 mcg dose equals 20 units. For a 5 mg vial with 2 mL BAC water: concentration is 2.5 mg/mL, or 2.5 mcg per uL. A 50 mcg dose equals 20 uL (2 units). A 100 mcg dose equals 40 uL (4 units). Aliquot into single-use volumes immediately after reconstitution. SP is sensitive to oxidation and protease contamination. Do not store reconstituted solution beyond 7 days at 2 to 8 degrees Celsius. Avoid freeze-thaw cycles entirely.

Dosing based on Very limited human dosing data: extrapolated from research studies — 13 published references.View all sources →

How the Substance P Math Works

2mg in 2mL: =1mg/mL
1mg/mL: =10mcg per unit
50mcg / 10: =5 units
2mg / 50mcg: =40 doses

Based on a 2mg vial, 2mL of bacteriostatic water, the beginner dose (50mcg), and a U-100 syringe. Change any input in the calculator and every line moves with it.

Verify your calculation

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Substance P Cost

Prices are estimates and vary by source, location, and prescription status.Full pricing breakdown →

Substance P Pharmacokinetics

Pharmacokinetics — Active Dose Over Time

t½ = ~1-2 minutes (rapid enzymatic degradation)

After 1 half-life (2m): 50% remainsAfter 2 half-lives (3m): 25% remainsAfter 3 half-lives (5m): 12.5% remains

At a 100mcg dose: 50% = 50mcg remaining after 2m. Recommended frequency: Single dose (local injection near wound site).

Disclaimer: This curve is a simplified first-order exponential decay model. Actual pharmacokinetics vary based on injection site, individual metabolism, body composition, and other factors. Half-life values are approximate and based on available preclinical and clinical literature. Many research peptides lack formal human pharmacokinetic studies. This is for educational purposes only — not medical advice.

Read the full Substance P guide →