Not medical advice. Talk to your provider before using any peptide.
Full disclaimerPeptide Schedule Research TeamReviewed Apr 20269 Citations
Substance P11 residuesRPKPQQFFGLMEach bubble = one amino acid. Size = residue mass. Color = chemical class.Turns your vial size, BAC water, and dose into exact syringe units. Free, no signup.
Adjust vial, water, and dose — answer updates live
No source establishes this dose. The largest published human exposure is 150 pmol given intradermally, about 0.2 mcg, as a challenge test to raise a wheal (PMID 37547990). This tier is roughly 250 times that amount.
All three take the same units for the same dose. A smaller barrel just spreads it over more space.
on a U-100 syringe for a 50mcg dose
Add 2 mL BAC water to your 2 mg vial. Draw to 5.0 units on a U-100 syringe for a 50mcg dose. This vial will last 40 doses.
Copy link saves these numbers in the address. Anyone who opens it sees the same draw.
There is no repeating self-administered schedule to plan for it, so the protocol builder leaves it out. The calculator above still works out the draw. Read the Substance P profile
Substance P activates NK1R to mobilize bone marrow stem cells and stimulate wound repair in animal models (Kim et al., Nature Medicine, PMID 19270709). A tissue half-life of 1 to 2 minutes and zero human wound healing trials limit it to preclinical research settings.
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| Level | Dose / Injection | Frequency |
|---|---|---|
| Beginner | 50mcgNo source establishes this dose. The largest published human exposure is 150 pmol given intradermally, about 0.2 mcg, as a challenge test to raise a wheal (PMID 37547990). This tier is roughly 250 times that amount. | Single dose (local injection near wound site) |
| Moderate | 100mcgNo source establishes this dose. The largest published human exposure is 150 pmol given intradermally, about 0.2 mcg, as a challenge test to raise a wheal (PMID 37547990). This tier is roughly 500 times that amount. | Single dose (local injection near wound site) |
| Aggressive | 200mcgNo source establishes this dose. The largest published human exposure is 150 pmol given intradermally, about 0.2 mcg, as a challenge test to raise a wheal (PMID 37547990). This tier is roughly 990 times that amount. | Single dose (local injection near wound site) |
Start with two practical realities. First: Substance P is not sold by consumer peptide vendors. You need a research-grade source (Sigma-Aldrich S6883 or Tocris #1156), and most require institutional purchasing credentials. Second: the 1 to 2 minute tissue half-life means distant subcutaneous injection (abdomen for a knee wound, for example) is pharmacologically pointless. Inject directly at or immediately adjacent to the target tissue. Reconstitution math for a 2 mg vial: add 2 mL bacteriostatic water. Concentration equals 1 mg/mL, or 1 mcg per uL. A 50 mcg dose equals 50 uL, which is 5 units on a U-100 insulin syringe. A 100 mcg dose equals 10 units. A 200 mcg dose equals 20 units. For a 5 mg vial with 2 mL BAC water: concentration is 2.5 mg/mL, or 2.5 mcg per uL. A 50 mcg dose equals 20 uL (2 units). A 100 mcg dose equals 40 uL (4 units). Aliquot into single-use volumes immediately after reconstitution. SP is sensitive to oxidation and protease contamination. Do not store reconstituted solution beyond 7 days at 2 to 8 degrees Celsius. Avoid freeze-thaw cycles entirely.
Dosing based on No human therapeutic dosing exists. Published human exposure is intradermal challenge testing at 5 to 150 pmol, about 0.007 to 0.2 mcg (PMID 37547990). The tiers here are not established by any source, and every citation on this page is animal or review work. — 13 published references.View all sources →
Based on a 2mg vial, 2mL of bacteriostatic water, the beginner dose (50mcg), and a U-100 syringe. Change any input in the calculator and every line moves with it.
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Disclaimer: This curve is a simplified first-order exponential decay model. Actual pharmacokinetics vary based on injection site, individual metabolism, body composition, and other factors. Half-life values are approximate and based on available preclinical and clinical literature. Many research peptides lack formal human pharmacokinetic studies. This is for educational purposes only — not medical advice.
