How many units of PE-22-28 should I draw?

It depends on your vial size, BAC water, and dose. For a 5mg vial with 2mL BAC water, a beginner dose of 100mcg is typically 4 units on a U-100 syringe. Use the calculator above for your exact numbers.

How much BAC water should I add to PE-22-28?

The standard amount is 2mL of bacteriostatic water for a 5mg vial. More water gives a more dilute solution — easier to measure small doses. Less water means fewer injections per vial. Adjust the BAC water slider in the calculator to see how it changes your syringe units.

How long does a PE-22-28 vial last?

At a beginner dose of 100mcg daily, a 5mg vial lasts about 50 doses. The calculator shows exact doses per vial for your selected tier.

What syringe should I use for PE-22-28?

Most people use a U-100 insulin syringe (1mL / 100 units). For very small doses, a U-50 or U-30 syringe gives better precision. The calculator adjusts units automatically for whichever syringe you pick.

PE-22-28 Dosage Calculator

CognitiveInjection/NasalResearch~23 hours half-life

PE-22-28 is a synthetic heptapeptide (7 amino acids) derived from spadin, a naturally occurring fragment of the sortilin propeptide.

Rapid antidepressant-like effects in animal models (within 4 days)Stimulates hippocampal neurogenesis and BDNF expressionPotent TREK-1 channel blockade (IC50 0.12 nM)Extended duration of action (~23 hours vs. 7 hours for spadin)4 weeks on / 4 weeks off

Vial Size

BAC Water (mL)

Dosing Level

100mcg · Daily

Custom Dose (mcg) — optional

Syringe Type

4.0 units

100 units (1mL)

Concentration

2,500

mcg/mL

Draw Volume

0.040

Syringe Units

4.0

units

Doses / Vial

doses

Summary: Add 2mL BAC water to your 5mg vial. Draw to 4.0 units on a U-100 syringe for a 100mcg dose. This vial will last 50 doses.

Cycle Planner

Cycle Length (weeks)

Price Per Vial ($) — optional

Subcutaneous or Intranasal. Typical beginner frequency: daily.

PE-22-28 Pharmacokinetics

Pharmacokinetics — Active Dose Over Time

t½ = ~23 hours (functional, animal data)

After 1 half-life (23h): 50% remainsAfter 2 half-lives (46h): 25% remainsAfter 3 half-lives (3d): 12.5% remains

At a 200mcg dose: 50% = 100mcg remaining after 23h. Recommended frequency: Daily.

Disclaimer: This curve is a simplified first-order exponential decay model. Actual pharmacokinetics vary based on injection site, individual metabolism, body composition, and other factors. Half-life values are approximate and based on available preclinical and clinical literature. Many research peptides lack formal human pharmacokinetic studies. This is for educational purposes only — not medical advice.

PE-22-28 Dosing Protocol

Level	Dose / Injection	Frequency
Beginner	100mcg	Daily
Moderate	200mcg	Daily
Aggressive	400mcg	Daily

Note: Shortened spadin analog. TREK-1 potassium channel blocker (IC50 0.12 nM). Derived from sortilin propeptide. Animal research only — no human trials. Intranasal preferred for CNS access.

About PE-22-28

PE-22-28 is a synthetic heptapeptide (7 amino acids) derived from spadin, a naturally occurring fragment of the sortilin propeptide. It acts as a potent blocker of TREK-1 two-pore potassium channels in the brain, with an IC50 of 0.12 nM — roughly 300-fold more potent than the parent compound spadin. In animal models, PE-22-28 produces rapid antidepressant-like effects within 4 days of treatment, compared to the 3-4 weeks typical of SSRIs. It stimulates hippocampal neurogenesis, increases BDNF expression, and modulates serotonergic signaling. PE-22-28 also shows improved in vivo stability over spadin, with a functional duration of approximately 23 hours versus 7 hours. Preclinical stroke research has demonstrated neuroprotective effects, including reduced infarction size and improved motor and cognitive recovery. All current evidence comes from rodent studies — there are no published human clinical trials. This peptide remains strictly a research tool and should be approached with appropriate caution given the absence of human safety data.

View full PE-22-28guide →Add to schedule →