How many units of Oxytocin should I draw?

It depends on your vial size, BAC water, and dose. For a 1IU vial with 2mL BAC water, a beginner dose of 20IU is typically 4 units on a U-100 syringe. Use the calculator above for your exact numbers.

How much BAC water should I add to Oxytocin?

The standard amount is 2mL of bacteriostatic water for a 1IU vial. More water gives a more dilute solution — easier to measure small doses. Less water means fewer injections per vial. Adjust the BAC water slider in the calculator to see how it changes your syringe units.

How long does a Oxytocin vial last?

At a beginner dose of 20IU 3-5x/week (prn), a 1IU vial lasts about 50 doses. The calculator shows exact doses per vial for your selected tier.

What syringe should I use for Oxytocin?

Most people use a U-100 insulin syringe (1mL / 100 units). For very small doses, a U-50 or U-30 syringe gives better precision. The calculator adjusts units automatically for whichever syringe you pick.

How should I store Oxytocin after reconstitution?

Store reconstituted Oxytocin at 2-8°C (36-46°F) after reconstitution. Refrigerate at 2-8°C, use within 3-4 weeks. Before reconstitution: room temperature up to 6 months, refrigerated up to 2 years.

Does the dose actually matter for behavioral effects?

Yes. Clinical trials mostly use 20-40 IU per session. Below 20 IU, effects are likely sub-threshold for most people. Above 40 IU, some users report paradoxical anxiety or emotional flatness, possibly from over-activation of inhibitory interneurons. The sweet spot from research is that 20-40 IU range.

Not medical advice. Talk to your provider before using any peptide.

Full disclaimer

Peptide Schedule Research TeamReviewed Apr 20266 Citations

Sexual HealthFDA ApprovedGrade A

Sexual HealthMood RegulationAnxiety Management

Oxytocin Dosage Calculator

3 min half-life·Injection/Nasal·4w on / 4w off

Oxytocin is a nine-amino-acid neuropeptide with FDA approval for labor induction (Pitocin) and a large community evidence base for social anxiety and bonding. Clinical trials use 20 to 40 IU intranasal. Effects kick in around 30 minutes. Receptor downregulation limits daily use.

Popular100–300+1,468High

10 referencesVerified 2026-04-07

Vial Size

BAC Water (mL)

Dosing Level

20mcg · 3-5x/week (PRN)

Custom Dose (mcg) — optional

Syringe Type

4.0 units

100 units (1mL)

Concentration

500

mcg/mL

Draw Volume

0.040

Syringe Units

4.0

units

Doses / Vial

doses

Summary: Add 2mL BAC water to your 1mg vial. Draw to 4.0 units on a U-100 syringe for a 20mcg dose. This vial will last 50 doses.

Schedule This Protocol

20mcg 3-5x/week (prn) — draw 4.0 units on U-100 syringe

Intranasal / Subcutaneous. Typical beginner frequency: 3-5x/week (prn).At beginner dose (20IU) with a 1IU/2mL vial: draw to the 4-unit mark on a U-100 syringe.

After reconstituting, store at 2-8°C (36-46°F) after reconstitution (refrigerate at 2-8°c, use within 3-4 weeks).

View side effects and safety warnings →

Quick-Start: Reconstitution

1Wash hands. Remove the cap from the nasal spray device.
2If first use, prime the pump by pressing 5 to 10 times until a fine mist appears. Point away from face during priming.
3Close one nostril with a finger. Insert the spray tip into the open nostril. Tilt your head slightly forward.

Full injection guide →

Oxytocin Dosing Protocol

Clinical TrialClinical trials: 20-40 IU intranasal per session (multiple RCTs)

Level	Dose / Injection	Frequency
Beginner	20 IU	3-5x/week (PRN)
Moderate	24 IU	3-5x/week (PRN)
Aggressive	40 IU	3-5x/week (PRN)

Note:

The dosing unit here is IU, not mcg. The conversion: 1 IU equals approximately 1.68 mcg. Most compounded intranasal sprays deliver 4 IU per puff from a standard metered-dose device. For a 20 IU dose, that is 5 puffs total, alternating nostrils (3 left, 2 right, or vice versa). For 24 IU, you need 6 puffs. Prime the spray device before first use with 5 to 10 actuations pointed away from your face. A typical compounded bottle holds 100 IU/mL in a 10 mL bottle (1,000 IU total). At 20 IU per session, 3 times weekly, one bottle lasts about 50 sessions or roughly 17 weeks. Tilt your head slightly forward when spraying. Breathe in gently through the nose. Don't blow your nose for 10 minutes after. Nasal congestion kills absorption, so clear your sinuses first. Refrigerate after opening. Use within 3 to 4 weeks. The 4-weeks-on, 4-weeks-off cycle is not optional if you want sustained effects; daily users consistently report tolerance onset by week 3.

Dosing based on Clinical trials: 20-40 IU intranasal per session (multiple RCTs) — 10 published references.View all sources →

Verify your calculation

Cross-check your Oxytocin reconstitution math with AI

Oxytocin Cost

Brand Price

Pitocin (IV, obstetric): ~$20-60/vial in hospital setting: not applicable for behavioral off-label use

Compounded

Compounded intranasal (503A Rx): ~$30-80/bottle (100-200 IU total, typically 100 IU/mL in 10 mL bottle; ~$0.30-0.80/IU). Research-grade vendor vials: ~$30-50 (quality unverified). January 2025 FDA compounding guidance may affect availability.

Pricing updated 2026-04-09

Prices are estimates and vary by source, location, and prescription status.Full pricing breakdown →

Oxytocin Pharmacokinetics

Pharmacokinetics — Active Dose Over Time

t½ = ~3 min (IV), central effects last 2-4 hours (intranasal)

After 1 half-life (3h): 50% remainsAfter 2 half-lives (6h): 25% remainsAfter 3 half-lives (9h): 12.5% remains

At a 24mcg dose: 50% = 12mcg remaining after 3h. Recommended frequency: 3-5x/week (PRN).

Disclaimer: This curve is a simplified first-order exponential decay model. Actual pharmacokinetics vary based on injection site, individual metabolism, body composition, and other factors. Half-life values are approximate and based on available preclinical and clinical literature. Many research peptides lack formal human pharmacokinetic studies. This is for educational purposes only — not medical advice.