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Not medical advice. Talk to your provider before using any peptide. Full disclaimer

Peptide Schedule Research TeamReviewed Apr 7, 20269 Citations

PNC-27 peptide iconPNC-2731 residues (approx.)PQHLRVKRPQHLRVRQIKIWFQNRRMKWKKEach bubble = one amino acid. Size = residue mass. Color = chemical class.Uses closest standard amino acids for non-standard residues.

PNC-27 Dosage Calculator

Immune · Research · Grade D · ~2-4 hours half-life (estimated) · Injection

Calculate your dose

Adjust vial, water and dose. The answer updates live.

mL
Dose
Syringe

All three take the same units for the same dose. A smaller barrel just spreads it over more space.

↓ 4.0020406080100
Draw to 4.0 units= 0.040 mL

on a U-100 syringe for a 100 mcg = 0.1 mg dose

Check that your syringe says U-100.

Concentration
2,500
mcg/mL
Doses / vial
50
Cost / dose
Your mixing recipe

Add 2 mL BAC water to your 5 mg vial. Draw to 4.0 units on a U-100 syringe for a 100 mcg dose. This vial will last 50 doses.

A reminder for 100 mcg (4 units) every day, with a free account.

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What you need to mix this6 items, matched to your vial, BAC water and syringe
  • PNC-27, 5 mg vialThe vial size set in the calculator.
  • Bacteriostatic water, 2 mLPrescription-only in the US, so it is dispensed by a pharmacy on a clinician's prescription.
  • 1 mL U-100 insulin syringe (100 units)Matches the syringe set in the calculator. Use a new sterile one for every injection.
  • Alcohol swabsFor each vial stopper every time a needle goes in, and for the injection site. Let the alcohol dry first.
  • A sharps containerUsed syringes go in right after each injection, never loose in the trash.
  • Storage after mixingRefrigerate at 2-8°C, use within 7-10 days.

How to choose each item: the peptide supplies guide.

Open PNC-27 details
Side effects, protocols by goal, storage, and the sources behind these dosing numbers.
Ask AI about this mathPNC-27 profile

About PNC-27

Turns your vial size, BAC water, and dose into exact syringe units. Free to use.

PNC-27 kills cancer cells by forming 34 nm membrane pores after binding surface HDM-2. Active against breast, pancreatic, leukemia, and ovarian lines in preclinical models. Zero human trials exist. FDA issued a 2017 contamination warning. Research-only compound with a safety grade of D.

AnticancerHDM-2 TargetingMembrane Pore FormationNecroticp53-Derived
Subcutaneous. Typical beginner frequency: daily.At beginner dose (100 mcg) with a 5mg/2mL vial: draw to the 4-unit mark on a U-100 syringe.
After reconstituting, store at -20°C lyophilized; 2-8°C (36-46°F) after reconstitution. Refrigerate at 2-8°C, use within 7-10 days.

View side effects and safety warnings →

Quick-Start: Reconstitution

  1. 1Pull a 5 mg or 10 mg vial of lyophilized PNC-27 from the freezer (-20 degrees C). Let it reach room temperature before reconstituting.
  2. 2Draw 2 mL of bacteriostatic water into a syringe. Inject slowly down the inside wall of the vial. Swirl gently until dissolved. Don't shake.
  3. 3Calculate your dose. With a 5 mg vial in 2 mL BAC water (2,500 mcg/mL): 100 mcg = 4 units, 250 mcg = 10 units, 500 mcg = 20 units on a U-100 insulin syringe. With a 10 mg vial: 100 mcg = 2 units, 250 mcg = 5 units, 500 mcg = 10 units.
Full injection guide →
Need a different vial size? Open the universal PNC-27 reconstitution calculator to enter any vial mg + BAC water mL.

PNC-27 Dosing Protocol

SpeculativeVery limited human data: dose based on preclinical research
LevelDose / InjectionFrequency
Beginner100 mcgDaily
Moderate250 mcgDaily
Aggressive500 mcgDaily
Note:

All dosing is theoretical. Zero human PK data exists for PNC-27. The numbers below come from preclinical IC50 extrapolation and a single murine MTD study.

Reconstitution math for a 5 mg vial with 2 mL bacteriostatic water: concentration is 2,500 mcg/mL, so 100 mcg equals 4 units on a U-100 insulin syringe, 250 mcg equals 10 units, and 500 mcg equals 20 units. For a 10 mg vial with 2 mL BAC water: concentration is 5,000 mcg/mL; 100 mcg equals 2 units, 250 mcg equals 5 units, 500 mcg equals 10 units.

Swirl the vial gently after adding water. Don't shake it. Peptides are fragile.

The thing most people miss: standard apoptosis assays (Annexin V, caspase panels) will give false negatives because PNC-27 kills by necrosis. Use LDH release or propidium iodide exclusion if you're testing cytotoxicity in a research setting.

Short half-life means daily dosing is necessary. Reconstituted solution goes in the fridge and should be discarded after 7 days. Lyophilized powder stores at -20 degrees C.

Vial quality is a real risk. The FDA found bacterial contamination in a commercial PNC-27 product in 2017. Only third-party sterility testing from an accredited lab checks for that; a purity certificate alone does not.

Dosing based on Very limited human data: dose based on preclinical research: 9 published references.View all sources →

How the PNC-27 Math Works

5mg in 2mL
=2.5mg/mL
2.5mg/mL
=25 mcg per unit
100 mcg / 25
=4 units
5mg / 100 mcg
=50 doses

Based on a 5mg vial, 2mL of bacteriostatic water, the beginner dose (100 mcg), and a U-100 syringe. Change any input in the calculator and every line moves with it.

Ask AI about this math

Cross-check your PNC-27 reconstitution math with AI

PNC-27 Cost

Generic / Research
Gray-market research-grade product (Apr 2026): 2 mg = $69.99; 5 mg = $153.99 to $258.60 (TFA-free, lab reagent); 10 mg = $219.99. Approximate range: $35/mg (bulk) to $52/mg (TFA-free).

Pricing updated 2026-04-09

Prices are estimates and vary by source, location, and prescription status.Full pricing breakdown →

PNC-27 Pharmacokinetics

Pharmacokinetics: Active Dose Over Time

t½ = ~2-4 hours (estimated from in vitro decay; no formal human PK studies)
50%25%12.5%100%75%50%25%0%03h6h9h12h15hTime after injectionDose remaining
After 1 half-life (3h): 50% remainsAfter 2 half-lives (6h): 25% remainsAfter 3 half-lives (9h): 12.5% remains
At a 250 mcg dose: 50% = 125 mcg remaining after 3h. Moderate-tier frequency in our sources: Daily.

Disclaimer: This curve is a simplified first-order exponential decay model. Actual pharmacokinetics vary based on injection site, individual metabolism, body composition, and other factors. Half-life values are approximate and based on available preclinical and clinical literature. Many research peptides lack formal human pharmacokinetic studies. This is for educational purposes only, not medical advice.

Read the full PNC-27 guide →

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Frequently Asked Questions

It depends on your vial size, BAC water, and dose. For a 5mg vial with 2mL BAC water, a beginner dose of 100mcg is typically 4 units on a U-100 syringe. Use the calculator above for your exact numbers.

The standard amount is 2mL of bacteriostatic water for a 5mg vial. More water gives a more dilute solution, so the same dose fills more of the syringe and is easier to measure. Less water makes the draw smaller and harder to read. The number of doses per vial does not change either way, since that depends only on vial size and dose. Adjust the BAC water slider in the calculator to see how it changes your syringe units.

At a beginner dose of 100mcg daily, a 5mg vial lasts about 50 doses. The calculator shows exact doses per vial for your selected tier.

Most people use a 1mL U-100 insulin syringe (100 units). For very small doses, a 0.5mL or 0.3mL barrel is easier to read. All three are U-100 graduated, so the number of units to draw is the same on each. Veterinary U-40 syringes are a different scale and the calculator adjusts for that separately.

Store reconstituted PNC-27 at -20°C lyophilized; 2-8°C (36-46°F) after reconstitution. Refrigerate at 2-8°C, use within 7-10 days. Unopened vials: Store at -20°C (freezer) for long-term stability; stable at 2-8°C for up to 2 months.