How much BAC water should I add?

It depends on your vial size and desired dose concentration. Use our reconstitution calculator to determine the exact amount. Common starting points: 2mL for a 5mg vial, 1mL for a 2mg vial.

Can I use sterile water instead of BAC water?

Technically yes, but BAC water is strongly preferred. Sterile water lacks the preservative (benzyl alcohol) that prevents bacterial growth in multi-use vials. With sterile water, you must use the entire vial within 24 hours.

What if I shake the vial?

Gentle shaking is usually fine. Vigorous shaking can denature the peptide (damage its structure). If you accidentally shook it hard, let it settle and check that the solution is clear. A foamy or cloudy solution may indicate degradation.

How to Reconstitute Peptides

Step-by-step guide to properly mixing your peptide powder with bacteriostatic water for injection.

What is Reconstitution?

Reconstitution is the process of mixing lyophilized (freeze-dried) peptide powder with bacteriostatic water to create an injectable solution. Peptides are shipped as powder because they're more stable in this form. Once reconstituted, the solution must be refrigerated and used within 3-4 weeks.

What You Need

Lyophilized peptide vial, bacteriostatic water (BAC water — sterile water with 0.9% benzyl alcohol preservative), insulin syringe for mixing, alcohol swabs, and a clean workspace. Never use plain sterile water — it lacks the preservative needed for multi-use vials.

Step 1: Clean Everything

Wash your hands thoroughly. Wipe the tops of both the peptide vial and the BAC water vial with alcohol swabs. Let the alcohol dry completely before proceeding. Work on a clean, flat surface.

Step 2: Draw the Water

Using a fresh insulin syringe, draw the desired amount of BAC water. Common amounts are 1mL or 2mL depending on the peptide. Our calculator will tell you the optimal amount for your specific peptide and desired concentration.

Step 3: Add Water to the Vial

Insert the needle through the rubber stopper of the peptide vial. Angle the syringe so water runs down the inside wall of the vial — do NOT squirt water directly onto the powder cake. Release the water slowly. The peptide is delicate and vigorous mixing can damage it.

Step 4: Let It Dissolve

Do NOT shake the vial. Instead, gently swirl it in small circles or simply let it sit for a few minutes. The powder should dissolve into a clear solution within 5-10 minutes. If particles remain after 10 minutes, continue gentle swirling. If the solution is cloudy, it may indicate degradation.

Step 5: Store Properly

Once reconstituted, immediately refrigerate the vial at 2-8°C (36-46°F). Keep it away from light. Most reconstituted peptides are stable for 3-4 weeks when refrigerated with BAC water. Label the vial with the reconstitution date.

Frequently Asked Questions

Try the Dosage Calculator Reconstitution Calculator