Peptide Schedule
Humanin24 residuesMAPRGFSCLLLLTSEIDLPVKRRAEach bubble = one amino acid. Size = residue mass. Color = chemical class.Humanin Dosage Calculator
Humanin belongs to a small but growing class of mitochondrial-derived peptides — bioactive molecules encoded directly in mitochondrial DNA rather than the nuclear genome.
1mcg · EOD
Summary: Add 2mL BAC water to your 2mg vial. Draw to 0.1 units on a U-100 syringe for a 1mcg dose. This vial will last 2000 doses.
Cycle Planner
Humanin Pharmacokinetics
Pharmacokinetics — Active Dose Over Time
t½ = ~30 min in mice (IP); >4 hours in rats (IP). Human half-life unknown.Disclaimer: This curve is a simplified first-order exponential decay model. Actual pharmacokinetics vary based on injection site, individual metabolism, body composition, and other factors. Half-life values are approximate and based on available preclinical and clinical literature. Many research peptides lack formal human pharmacokinetic studies. This is for educational purposes only — not medical advice.
Humanin Dosing Protocol
| Level | Dose / Injection | Frequency |
|---|---|---|
| Beginner | 1mg | EOD |
| Moderate | 3mg | 3x/week |
| Aggressive | 5mg | 3x/week |
Note: Humanin is a 24-amino acid peptide encoded within the 16S ribosomal RNA gene of mitochondrial DNA. It was first discovered in 2001 from the occipital lobe of an Alzheimer's patient. It's one of several mitochondrial-derived peptides (MDPs), placing it in the same family as MOTS-c. The most studied analog is HNG (S14G-Humanin), which is roughly 1,000x more potent than native humanin in cell-based assays. Nearly all data comes from in vitro and animal models. There are no completed human clinical trials.
About Humanin
Humanin belongs to a small but growing class of mitochondrial-derived peptides — bioactive molecules encoded directly in mitochondrial DNA rather than the nuclear genome. Discovered in 2001 by Nishimoto and colleagues, it was isolated from surviving neurons in the brain of an Alzheimer's disease patient. That origin story set the stage for two decades of research into its neuroprotective and anti-aging potential. The peptide is just 24 amino acids long, but it packs a surprising amount of biological activity. In cell culture and animal models, humanin protects neurons from amyloid-beta toxicity, reduces oxidative stress, and inhibits programmed cell death through multiple pathways. It directly binds the pro-apoptotic protein BAX, preventing it from puncturing mitochondrial membranes. It also interacts with IGFBP-3 to block IGF-1-independent apoptosis, and it activates STAT3 signaling through a trimeric receptor complex. Circulating humanin levels decline with age, which has prompted speculation that it plays a role in the aging process itself. Mouse studies show that humanin administration extends healthspan metrics and improves insulin sensitivity. A 2020 study in Aging found that humanin-treated mice showed better cognitive performance, improved metabolic markers, and reduced inflammatory signaling compared to controls. The HNG analog (S14G substitution) is the most commonly used research variant because it's up to 1,000-fold more potent than native humanin. Most animal dosing studies use HNG rather than the wild-type peptide. That said, humanin research is still overwhelmingly preclinical. No human clinical trials have been completed, and its pharmacokinetic profile in people hasn't been established. The cancer safety question also remains open — humanin's anti-apoptotic effects could theoretically protect tumor cells, and some animal data supports this concern.