How many units of ACE-031 should I draw?

It depends on your vial size, BAC water, and dose. For a 1mg vial with 1mL BAC water, a beginner dose of 500mcg is typically 50 units on a U-100 syringe. Use the calculator above for your exact numbers.

How much BAC water should I add to ACE-031?

The standard amount is 1mL of bacteriostatic water for a 1mg vial. More water gives a more dilute solution, so the same dose fills more of the syringe and is easier to measure. Less water makes the draw smaller and harder to read. The number of doses per vial does not change either way, since that depends only on vial size and dose. Adjust the BAC water slider in the calculator to see how it changes your syringe units.

How long does a ACE-031 vial last?

At a beginner dose of 500mcg every 2-4 weeks (trials halted for safety), a 1mg vial lasts about 2 doses. The calculator shows exact doses per vial for your selected tier.

What syringe should I use for ACE-031?

Most people use a 1mL U-100 insulin syringe (100 units). For very small doses, a 0.5mL or 0.3mL barrel is easier to read. All three are U-100 graduated, so the number of units to draw is the same on each. Veterinary U-40 syringes are a different scale and the calculator adjusts for that separately.

How should I store ACE-031 after reconstitution?

Store reconstituted ACE-031 at -20°C (lyophilized); 2-8°C (reconstituted). Refrigerate at 2-8°C after reconstitution. Use within 14-21 days. Do not freeze reconstituted solution. Protect from light.. Before reconstitution: store at -20°c or colder. stable for 12-24 months when kept dry and protected from light. do not subject to repeated freeze-thaw cycles..

Why does the community dose so much lower than clinical trials?

Clinical trials used 0.5–3 mg/kg in patients with diagnosed muscle-wasting disease under supervision. Phase 1 data showed lean mass responses starting at 0.3 mg/kg, so community users target that low end; roughly 100–500 mcg for typical body weights rather than the 35–210 mg used clinically.

Not medical advice. Talk to your provider before using any peptide.

Full disclaimer

Peptide Schedule Research TeamReviewed Apr 20266 Citations

ACE-031 Dosage Calculator

Turns your vial size, BAC water, and dose into exact syringe units. Free, no signup.

Growth HormoneResearchGrade C·10-15 days half-life·Injection·12w on / 10w off

8 refsVerified 2026-04-07

Calculate your dose

Adjust vial, water, and dose — answer updates live

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Vial Size

BAC Water (mL)

Dose Tier

500mcg · Every 2-4 weeks (trials halted for safety)

Custom Dose (mcg) optional

Syringe

All three take the same units for the same dose. A smaller barrel just spreads it over more space.

Draw to 50.0 units= 0.500 mL

on a U-100 syringe for a 500mcg dose

Concentration

1,000

mcg/mL

Doses / vial

Cost / dose

Your mixing recipe

Add 1 mL BAC water to your 1 mg vial. Draw to 50.0 units on a U-100 syringe for a 500mcg dose. This vial will last 2 doses.

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Never miss a dose — 500mcg every 2-4 weeks (trials halted for safety), draw 50.0 units on U-100 syringe.

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Save this as a tracked protocol

Never miss a dose — 500mcg every 2-4 weeks (trials halted for safety), draw 50.0 units on U-100 syringe.

Calendar sync

Apple · Google · Outlook

Daily reminders

Never miss a dose

Dose tracking

See adherence streaks

Cycle planning

Auto on/off weeks

Tracking needs a free account, about 20 seconds
The calculator stays free without one

Cycle Length (weeks)

Price Per Vial ($) — optional

Open ACE-031 details

Side effects, protocols by goal, storage, and the sources behind these dosing numbers.

About ACE-031

ACE-031 traps myostatin to promote lean mass gains. Phase 1 showed 3.3% muscle increase from one injection, but vascular safety signals halted clinical development. Community protocols use doses far below trial levels. Quality verification is difficult for this recombinant fusion protein.

Myostatin InhibitorActRIIBMuscle GrowthFusion ProteinDuchenne Muscular DystrophyLigand Trap

Niche50–1008Low

Subcutaneous. Typical beginner frequency: every 2-4 weeks (trials halted for safety).At beginner dose (500mcg) with a 1mg/1mL vial: draw to the 50-unit mark on a U-100 syringe.

After reconstituting, store at -20°C (lyophilized); 2-8°C (reconstituted) (refrigerate at 2-8°c after reconstitution. use within 14-21 days. do not freeze reconstituted solution. protect from light.).

View side effects and safety warnings →

Quick-Start: Reconstitution

1Reconstitute by adding bacteriostatic water slowly along the vial wall. For a 1 mg vial, use 1 mL. For a 5 mg vial, use 2 mL. Swirl gently until the powder dissolves completely. Never shake or vortex.
2Draw the calculated dose using an insulin syringe (29 to 31 gauge). For 500 mcg from a 1 mg/1 mL vial, draw to the 50 unit mark. For 500 mcg from a 5 mg/2 mL vial, draw to the 20 unit mark. For 1000 mcg from a 5 mg/2 mL vial, draw to the 40 unit mark. For 1500 mcg from a 5 mg/2 mL vial, draw to the 60 unit mark.
3Inject subcutaneously into the abdomen, upper thigh, or upper arm. Rotate injection sites between doses. With biweekly dosing, you have plenty of time for site recovery.

Full injection guide→

Need a different vial size? Open the universal ACE-031 reconstitution calculator to enter any vial mg + BAC water mL.

ACE-031 Dosing Protocol

ExtrapolatedDose from the Phase 1 single-ascending-dose study in healthy postmenopausal women (PMID 23169607); the every-2-to-4-week interval comes from the DMD Phase 2 program (PMID 27462804), which was halted for epistaxis and telangiectasia

Level	Dose / Injection	Frequency
Beginner	500mcg	Every 2-4 weeks (trials halted for safety)
Moderate	1mg	Every 2-4 weeks (trials halted for safety)
Aggressive	1.5mg	Every 2-4 weeks (trials halted for safety)

Note:

ACE-031 is a large fusion protein, not a standard peptide. Reconstitution matters more here than with most compounds. For a 1 mg vial with 1 mL bacteriostatic water: concentration is 1000 mcg/mL. A 500 mcg dose is 50 units on an insulin syringe. A 1000 mcg dose uses the full vial. For a 5 mg vial with 2 mL bacteriostatic water: concentration is 2500 mcg/mL. A 500 mcg dose is 20 units. A 1000 mcg dose is 40 units. A 1500 mcg dose is 60 units. Add water slowly along the vial wall. Do not shake. ACE-031 is an 82 kDa protein that can denature with aggressive mixing; swirl gently until clear. If the solution looks cloudy or has particles after reconstitution, do not inject it. Protein aggregation means the compound may be damaged or degraded. The single biggest concern with ACE-031 is product authenticity. Standard HPLC purity testing tells you the mass is correct, not that the protein is properly folded and glycosylated. Request SDS-PAGE gel data from your supplier. If they can't provide it, you're guessing.

Dosing based on Dose from the Phase 1 single-ascending-dose study in healthy postmenopausal women (PMID 23169607); the every-2-to-4-week interval comes from the DMD Phase 2 program (PMID 27462804), which was halted for epistaxis and telangiectasia — 8 published references.View all sources →

How the ACE-031 Math Works

1mg in 1mL: =1mg/mL
1mg/mL: =10mcg per unit
500mcg / 10: =50 units
1mg / 500mcg: =2 doses

Based on a 1mg vial, 1mL of bacteriostatic water, the beginner dose (500mcg), and a U-100 syringe. Change any input in the calculator and every line moves with it.

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ACE-031 Cost

Compounded

$30-200 per 1 mg vial from research chemical vendors (wide price range reflects quality uncertainty). SwissChems ~$29/mg, Real-Peptides ~$110/mg, Core Peptides ~$155-165/mg. Chinese bulk suppliers $20-150/unit. Effective cost for a 500 mcg biweekly protocol: ~$15-100/dose depending on source. For 1000 mcg: ~$30-200/dose. This is expensive relative to most peptides.

Pricing updated 2026-04-09

Prices are estimates and vary by source, location, and prescription status.Full pricing breakdown →

ACE-031 Pharmacokinetics

Pharmacokinetics — Active Dose Over Time

t½ = 10-15 days

After 1 half-life (13d): 50% remainsAfter 2 half-lives (25d): 25% remainsAfter 3 half-lives (38d): 12.5% remains

At a 1mg dose: 50% = 500mcg remaining after 13d. Recommended frequency: Every 2-4 weeks (trials halted for safety).

Disclaimer: This curve is a simplified first-order exponential decay model. Actual pharmacokinetics vary based on injection site, individual metabolism, body composition, and other factors. Half-life values are approximate and based on available preclinical and clinical literature. Many research peptides lack formal human pharmacokinetic studies. This is for educational purposes only — not medical advice.

Read the full ACE-031 guide →